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TRANSFORMATION OF tissue effects of low temperature of the freezing interface


A time. Because of the amount of the introduction of the following without a transform-type = Lu (of 10) are:. 272 + = o ...) differential equations (11) the solution is: = a ÷ (port a) +2 a (a-) a ln (a-) + c (12) integral constant C r 10 from the initial conditions, r-R, T = T, obtained by c = (port-1). A 2a (a-1) + port ln (a-1) (13) into (12) after: In twenty-one blindly (I 1) [port +1 (a 1)] population of a Z (14) as and time is proportional to r, and r is proportional to equation (14) essentially reflects the relationship between r and time. That expressed the freezing of biological tissue interface phase changing circumstances over time. O. 7; O. 4O. 1200400600800l0001200 Next / s in a 233K, T = 77K freezing conditions, phase change interface Shimoda 4 changes over time China Institute of Metrology Section 78 17 Figure 4 shows a 233K,[link widoczny dla zalogowanych], L TRANSFORMATION OF freezing conditions for a 77K time interface changes. When the freezing time l5 ~ 18rain, the phase change interface to freeze up to steady-state diameter of 8o% or so. Figure 4 Changes in the slope of dr / dr freeze the phase change interface reflects the rate of outward expansion, the variation in Figure 5. O-90.40.30.20l200400600800l000l200f / s = 233K. Tp = 77K,[link widoczny dla zalogowanych], 5 ring 6mm diameter probe frozen phase freezing interface growth rate variation with time 3 Conclusions 1) cryopreservation on cell and tissue damage and injury is due to intracellular and extracellular ice formation caused by ice , is the destruction of cell membrane structure and function of cell injury, and the microcirculation resulting in hypoxic cell injury caused by various factors such as the role of the results. 2) freezing cold to fully probe the case of frozen biological tissues at low temperatures under the action of the probe, freezing the phase change interface diameter in the steady state wall temperature and cryogenic probes, freezing the probe diameter,[link widoczny dla zalogowanych], and tissue lesions Anti required temperature. Cryogenic probe wall temperature is lower, the larger diameter phase freezing interface, the greater the area of ​​biological tissue freezing. 3) In the process of cryogenic surgery, due to the existence of such factors as blood supply, phase change interface outward expansion of the freezing rate decreases rapidly, usually 15 ~ 18rain freeze the phase change interface has reached a steady state of the 8O9, 6, thus freezing time too long, for increasing the scope of biological tissue freezing phase change is not meaningful. Clinical practice can be frozen for a repeated freeze thawing of a way to improve efficacy. 【References [1] Huang Jingxiang. Cryogenic technology in biomedical applications [J]. Cryogenics and Superconductivity. 1985 (1) 179-82. [2] Huang Jingxiang. Cold liquid nitrogen temperature treatment machine approximate calculation [J]. Cryogenics and Superconductivity, 1984 (3) 126-28. [3] HUA Ze-Zhao, Ren Hesheng. Cryobiology medical technology I-J]. T Science Press, Beijing. 1994135-58. r4] Shun-Hua Zhou. Liu Baolin. Coke rocks. A new low-temperature glass breaking technologies [J]. Cryogenic Engineering. 2000 (5): 21 ~ 24. [5] Zhang Zhong Min. Cold treatment and cold storage f-J]. Cryogenics and Superconductivity. 1986 (2) t31-35. [6] GAGEAA. Criticaltemperatureforskinnecrosisinexperimentalcryosurgery [J]. Cryobiology. 1982 (19) :273-281. [7] El,[link widoczny dla zalogowanych], a SHAKHSS, SHIM1S. BENSONEE. eta1. Physicalobservationsonrapidfreezingoftumourcellsto a 40 ℃ usingdifferenticalscanningcalorimetry [J]. Cryoletters. 1998 (19) :159-170. [8] Zhang Zhong Min. Mao Xinxin. He Tao. Frozen tissue around the probe and test the calculation of temperature distribution [J]. Cryogenics and Superconductivity. 1998 (4): 4O a 45. [93 HE Tao, Zhang Zhong-Min, Mao Xinxin. Cold cold calculation medical equipment and test methods IJ]. Cryogenics and Superconductivity,[link widoczny dla zalogowanych],】 999 (2), 42-47. [10] History of the British. Zhang Zhong-Min, Huang Jingxiang. And so on. Cryopreservation of biological material of the device [J]. Cryogenics and Superconductivity. 2005 (3); 5】 a 54.

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